Bmi1 (COMMD3-BMI1) Goat Polyclonal Antibody

CAT#: AP07560PU-N

Bmi1 (COMMD3-BMI1) goat polyclonal antibody, Aff - Purified

Product Images

Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/5000 - 1/30000.
  Immunocytochemistry.
  Immunofluorescence.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 1/500 - 1/3000.
• Reactivities: Human, Bovine, Canine, Chicken, Feline, Frog, Monkey, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide
• Specificity: This antibody reacts to DNA-binding protein Bmi-1.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide
  State: Aff - Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: COMMD3-BMI1 readthrough
• Database Link:
  Entrez Gene 100532731 Human
  UniProt ID P35226
• Background: The Bmi-1 oncogene (also known as polycomb group ring finger 4, MGC12685, murine leukemia viral (bmi 1) oncogene homolog, oncogene BMI 1, polycomb complex protein BMI 1 and RNF51) induces telomerase activity and immortalizes human mammary epithelial cells. Bmi-1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. The polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi-1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation.
• Synonyms: BMI1, PCGF4