14-3-3 zeta (YWHAZ) Rabbit Polyclonal Antibody

CAT#: AP06623PU-N

14-3-3 zeta (YWHAZ) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of 14-3-3 ?´ antibody at 1/500 dilution Lane 1:MCF-7 cell lysate Lane 2:Hela cell lysate Lane 3:sp2/0 cell lysate Lane 4:H9C2 cell lysate

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  Immunohistochemistry (IHC) analyzes of 14-3-3 ?¶/?´ antibody in paraffin-embedded human colon carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 200-250 of Human 14-3-3 ζ.
• Specificity: This antibody detects endogenous levels of 14-3-3 ζ/δ protein.
  (region surrounding Trp228)
• Formulation: Phosphate Buffered Saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~28 kDa
• Gene Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
• Database Link:
  Entrez Gene 7534 Human
  UniProt ID P63104
• Background: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.
• Synonyms: YWHAZ, KCIP-1