NFAT4 (NFATC3) Rabbit Polyclonal Antibody

CAT#: AP06545PU-N

NFAT4 (NFATC3) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of NFAT4 antibody in extracts from HeLa cells treated with Ca+ 40nM 30'.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 130-180 of Human NFAT4.
• Specificity: This antibody detects endogenous levels of NFAT4 protein.
  (region surrounding Glu159)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (>95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 115 kDa
• Gene Name: nuclear factor of activated T-cells 3
• Database Link:
  Entrez Gene 4775 Human
  UniProt ID Q12968
• Background: NFATc4 (nuclear factor of activated T cells, cytoplasmic, calcineurindependent 4) is a member of the nuclear factors of activated T cells DNAbinding transcription complex that influences cytokine gene expression, cardiac hypertrophy and adipocyte differentiation. This complex consists of at least two components, a cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate in the formation of this complex. NFATc4 plays a role in the inducible expression of cytokine genes in T cells, including the induction of IL-2 and IL-4. p38 MAP kinase phosphorylates multiple residues in the NFAT homology domain of NFATc4.
• Synonyms: NF-ATc3, NFATc3, T-cell transcription factor NFAT4