PIAS1 Rabbit Polyclonal Antibody

CAT#: AP06452PU-N

PIAS1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of PIAS1 antibody (Cat.-No.: AP06452PU-N) in extracts from Jurkat cells.

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  Immunohistochemistry (IHC) analyzes of PIAS1 antibody (Cat.-No.: AP06452PU-N) in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of PIAS1 protein.
  (region surrounding Gly24)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 72 kDa
• Gene Name: protein inhibitor of activated STAT 1
• Database Link:
  Entrez Gene 8554 Human
  UniProt ID O75925
• Background: The IL-6-type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.
• Synonyms: PIAS-1, DDXBP1, Gu-binding protein, RNA helicase II-binding protein, DEAD/H box-binding protein 1, Protein inhibitor of activated STAT protein 1