Caspase 8 (CASP8) Rabbit Polyclonal Antibody

CAT#: AP06376PU-N

Caspase 8 (CASP8) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Caspase 8 antibody

• 

  Immunohistochemistry (IHC) analyzes of Caspase 8 antibody in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 312-368 of Human Caspase-8.
• Specificity: This antibody detects endogenous levels of Caspase 8 protein.
  (region surrounding Thr341)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 38, 55 kDa
• Gene Name: caspase 8
• Database Link:
  Entrez Gene 841 Human
  UniProt ID Q14790
• Background: Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
• Synonyms: CASP-8, CASP8, MCH5, CAP4