EIF4EBP1 Rabbit Polyclonal Antibody

CAT#: AP06364PU-N

EIF4EBP1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of 4E-BP1 antibody in extracts from Jurkat cells treated with Insulin 0.01U/ml 15'.

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  Immunohistochemistry (IHC) analyzes of 4E-BP1 antibody in paraffin-embedded human colon carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 26-76 of Human 4E-BP1.
• Specificity: This antibody detects endogenous levels of 4E-BP1 protein.
  (region surrounding Leu58)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 15 to 20 kDa
• Gene Name: eukaryotic translation initiation factor 4E binding protein 1
• Database Link:
  Entrez Gene 1978 Human
  UniProt ID Q13541
• Background: The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E.
• Synonyms: PHAS-I, PHAS-1, PHAS1