PTCH1 Rabbit Polyclonal Antibody

CAT#: AP06278PU-N

PTCH1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Patched antibody at 1/500 dilution Lane 1:Hela whole cell lysate Lane 2:NIH-3T3 whole cell lysate Lane 3:PC12 whole cell lysate Lane 4:MCF-7 whole cell lysate

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  Immunohistochemistry (IHC) analysis of Patched antibody in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to the N-terminal of Human Patched.
• Specificity: This antibody detects endogenous levels of Patched protein.
  (region surrounding Ala2)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~160 kDa
• Gene Name: patched 1
• Database Link:
  Entrez Gene 5727 Human
  UniProt ID Q13635
• Background: PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). PTCH associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal.PTCH has a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. PTCH is expressed in the adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. It is also expressed in tumor cells but not in normal skin. During development PTCH is found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome
• Synonyms: PTCH, PTC1, PTC, Patched 1