MEK3 (MAP2K3) Rabbit Polyclonal Antibody

CAT#: AP06222PU-N

MEK3 (MAP2K3) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of MKK3 antibody in extracts from MDA-MB-435 cells

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  Immunohistochemistry (IHC) analyzes of MKK3 antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunuohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 150-200 of Human MEK-3.
• Specificity: This antibody detects endogenous levels of MKK3 protein.
  (region surrounding Ser183)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 39 kDa
• Gene Name: mitogen-activated protein kinase kinase 3
• Database Link:
  Entrez Gene 5606 Human
  UniProt ID P46734
• Background: The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinaseegulatory threonine and tyrosine residues present in thehr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6hosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.
• Synonyms: MAPKK 3, MEK3, MKK3, MAP kinase kinase 3