Cytokeratin 19 (KRT19) Rabbit Polyclonal Antibody

CAT#: AP06201PU-N

Cytokeratin 19 (KRT19) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Cytokeratin-19 Antibody in extracts from LOVO cells.

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  Immunohistochemistry analysis of Cytokeratin-19 Antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 221-270 of Human Cytokeratin 19.
• Specificity: This antibody detects endogenous levels of Cytokeratin 19 protein.
  (region surrounding Met259)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~44 kDa
• Gene Name: keratin 19
• Database Link:
  Entrez Gene 3880 Human
  UniProt ID P08727
• Background: Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratins assemble into filaments, forming heterodimers of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles, which make them useful as biomarkers (1). Mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
• Synonyms: Cytokeratin-19, Keratin-19, KRT19, CK19, K19