JUND Rabbit Polyclonal Antibody

CAT#: AP06193PU-N

JUND rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of JunD antibody in extracts from A549 cells.

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  Immunohistochemistry analysis of JunD antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunoprecipitation: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 220-270 of Human JunD.
• Specificity: This antibody detects endogenous levels of JunD protein.
  (region surrounding Val249)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~35.0 kDa
• Gene Name: JunD proto-oncogene, AP-1 transcription factor subunit
• Database Link:
  Entrez Gene 3727 Human
  UniProt ID P17535
• Background: The activator protein-1 (AP-1) transcription factor consists of either Jun/Jun homodimers or Fos/Jun heterodimeric complexes. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family. Studies suggest that the two forms of Jun D may be due to internal initiation of translation.
• Synonyms: JUND