JM4 (PRAF2) Rabbit Polyclonal Antibody

CAT#: AP06190PU-N

JM4 (PRAF2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of PRAF2 antibody at 1/500 dilution Lane 1:HEK293T whole cell lysate Lane 2:Rat brain tissue lysate Lane 3:Mouse brain tissue lysate

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  Immunohistochemistry (IHC) analysis of JM4/PRAF2 antibody in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffins Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 141-190 of Human PRAF2.
• Specificity: This antibody detects endogenous levels of JM4/PRAF2 protein.
  (region surrounding Leu166)
• Formulation: PBS, pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~20 kDa
• Gene Name: PRA1 domain family member 2
• Database Link:
  Entrez Gene 11230 Human
  UniProt ID O60831
• Background: JM4 (Jena-Muenchen 4), also known as PRAF2 (PRA1 domain family, member 2), is a 178 amino acid endosomal multi-pass membrane protein involved in vesicular trafficking and Endoplasmic reticulum/Golgi transport. As a member of the PRA1 family, JM4 contains four putative transmembrane (TM) domains, interacts with the CC chemokine receptor 5 (CCR5) and colocalizes with Calnexin in the ER and mannose 6-phosphate receptor (CD-MPR) in the Golgi apparatus. While ubiquitously expressed, JM4 has been found at high levels in small intestine, lung, pancreas, spleen, Purkinje cells of the cerebellum and in neuronal cells of the hippocampus, cerebral cortex and lateral ventricles of the brain. JM4 plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis and has been implicated in human cancer.
• Synonyms: PRA1 family protein 2