c-Jun (JUN) Rabbit Polyclonal Antibody

CAT#: AP06055PU-N

c-Jun (JUN) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of c-Jun antibody at 1/500 dilution Lane 1: Hela cell lysate Lane 2: NIH3T3 cell lysate Lane 3: Rat kidney tissue lysate

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  Immunohistochemical analysis using AP-1 / c-Jun antibody in Paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: Immunoprecipitation: 1/50-1/200.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
  Western blot: 1/500-1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 201-250 of Human c-Jun.
• Specificity: This antibody detects endogenous levels of AP-1 / c-Jun protein (region surrounding Gly237).
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~36, 43, 48 kDa
• Gene Name: Jun proto-oncogene, AP-1 transcription factor subunit
• Database Link:
  Entrez Gene 3725 Human
  UniProt ID P05412
• Background: The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein, along with c-Fos, is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D, have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.
• Synonyms: Transcription factor AP1