BRCA1 Rabbit Polyclonal Antibody

CAT#: AP06033PU-N

BRCA1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blo t (WB) analysis of BRCA1 antibody at 1/500 dilution Lane 1:Hela cell lysate Lane 2:Mouse kidney tissue lysate Lane 3:Rat brain tissue lysate

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BRCA1 antibody.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Immunohistochemistry on Paraffin sections: 1/50-1/200.
  Western blot: 1/500-1/1000.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 1500-1550 of Human BRCA1.
• Specificity: This antibody detects endogenous levels of BRCA1 protein (region surrounding Gln1518).
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 200 kDa
• Gene Name: BRCA1, DNA repair associated
• Database Link:
  Entrez Gene 672 Human
  UniProt ID P38398
• Background: BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full length natures identified.
• Synonyms: BRCAI; BRCC1; BROVCA1; IRIS; PSCP; RNF53