BIK Rabbit Polyclonal Antibody

CAT#: AP06031PU-N

BIK rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of BIK antibody in extracts from A549 cell treated with DMSO 0.1% 10'.

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  Immunohistochemical analysis in paraffin-embedded human lung carcinoma tissue using BIK antibody.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to the N-terminal of Human BIK.
• Specificity: The antibody detects endogenous levels of BIK protein (region surrounding Met27).
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography using epitope-specific immunogen (> 95% pure by SDS-PAGE).
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~23 kDa
• Gene Name: BCL2 interacting killer
• Database Link:
  Entrez Gene 638 Human
  UniProt ID Q13323
• Background: Bik/Nbk (Bcl-2-interacting killer/natural born killer) is a potent pro-apoptotic protein belonging to a group of Bcl-2 family members that includes Bad, Bid, Bim, Hrk, and Noxa, containing a BH3 domain but lacking other conserved domains, BH1 or BH2. Functionally, Bik is able to bind to and antagonize anti-apoptotic Bcl-2 family members including Bcl-2, Bcl-xL, and viral homologs E1B-19K and EBV-BHFR1. The BH3 domain of Bik is essential for its apoptotic activity and interaction with survival proteins. Phosphorylation of Bik is correlated with an increase in its pro-apoptotic activity.
• Synonyms: Bcl-2-interacting killer, NBK, BP4, BIP1