DARPP32 (PPP1R1B) pThr34 Rabbit Polyclonal Antibody

CAT#: AP05890PU-N

DARPP32 (PPP1R1B) pThr34 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 1:1000; detects a band of approximately 32kDa in rat caudate lysates.
• Reactivities: Bovine, Canine, Chicken, Human, Monkey, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within DARPP-32 which includes phosphorylated Thr34.
• Specificity: This antibody is specific for DARPP-32, when phosphorylated at threonine 34.
• Formulation: 10mM HEPES, pH7.5 containing 0.09% Sodium Azide, 0.1% Bovine Serum Albumin and 50% Glycerol
  State: Purified
  State: Liquid purified Ig
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein phosphatase 1 regulatory inhibitor subunit 1B
• Database Link:
  Entrez Gene 84152 Human
  UniProt ID Q9UD71
• Background: DARPP-32 (also known as protein phosphatase 1 regulatory subunit 1B PP1R1B) is a dopamine- and cAMP-regulated phosphoprotein, which is principally expressed in striatal medium spiny neurons, and is thought to play a critical role in the regulation of dopaminergic neurotransmission. DARPP-32 can act either as a phosphatase inhibitor or as a kinase inhibitor, depending on its relative state of phosphorylation . Phosphorylation at threonine 34 converts DARPP-32 into an inhibitor of protein phosphatase-1 (PP-1) whilst phosphorylation at threonine 75 switches the protein to an inhibitor of protein kinase A (PKA) .
• Synonyms: PPP1R1B, DARPP-32, FLJ20940