SPARC Rabbit Polyclonal Antibody

CAT#: AP05261SU-N

SPARC rabbit polyclonal antibody, Purified

Product Images

Detection of endogenous SPARC in mouse heart lysate using (5 ug/ml) and anti-rabbit HRP (1:75k) and using Pierce's Super Signal West Femto.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot: (1-5 µg/ml).
  Immunohistochemistry on Frozen Sections.
  Positive Control: Mouse heart lysate.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide derived from the Human SPARC protein
• Specificity: This antibody detects SPARC. It may Cross react with SPARCL1.
• Formulation: Phosphate buffered saline with 0.08% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Ammonium Sulfate Precipitation
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: secreted protein acidic and cysteine rich
• Database Link:
  Entrez Gene 6678 Human
  UniProt ID P09486
• Background: SPARC is a key factor in cell-matrix interactions and possibly tumour aggressiveness. The SPARC gene, which encodes a multifunctional glycoprotein with roles in tissue development, remodelling and fibrosis. A regulator of cell-extracellular matrix (ECM) interactions, SPARC represents a major factor in the ECM remodelling occurring during tumour invasion. in silico analysis reveals 4 UTR-SNPs located in the 3' UTR of the SPARC gene, corresponding to 1474 g a, 1551 g c, 1922 t g and 2072 c t changes, which are significantly associated with tumoral state of the tissue. Of all hits, the 2072 SPARC polymorphism had the best association with cancer.
  SPARC therefore is a gene involved in a number of diseases including rheumatoid arthritis, scleroderma, tumor development and metastasis. SPARC variants have been detected in tumour samples of patients with acute myeloblastic leukemia (AML).
• Synonyms: ON, Basement-membrane protein 40, BM-40