Chk1 (CHEK1) Sheep Polyclonal Antibody

CAT#: AP05017PU-N

Chk1 (CHEK1) sheep polyclonal antibody, Purified

Product Images

CHK1 antibody staining of cell lines at 1/500 dilution.

Specifications

Product Data

• Applications: IP, WB
• Recommended Dilution: Western Blot: 1-10 µg/ml.
  Immunoprecipitation.
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A GST fusion protein corresponding to amino acid sequence 1-474 with having a molecular weight of 54 kD.
• Specificity: This antibody recognizes CHK1 kinase.
• Formulation: Phosphate buffered saline with 0.08% Sodium Azide as preservative.
  State: Purified
  State: Liquid (0.2µm sterile filtered) purified IgG fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: checkpoint kinase 1
• Database Link:
  Entrez Gene 1111 Human
  UniProt ID O14757
• Background: One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic-dependent kinase cdc2, which regulates entry in to mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of cdc2. It is hypothesized that chk 1 induces serine 216 phosphorylation of Cdc 25C and subsequent binding negatively regulated Cdc25C, thus preventing it from activating cdc2.
• Synonyms: CHEK1, CHEK-1