Histone H2A.X (H2AFX) Rabbit Polyclonal Antibody

CAT#: AP02761PU-N

Histone H2A.X (H2AFX) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analyis of extracts from HepG2 and 293 cells using Histone H2A.X antibody

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  Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Histone H2A.X antibody.

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  Immunofluorescence staining of methanol-fixed HeLa cells using Histone H2A.X antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Immunofluoresence: 1/100 - 1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).
• Specificity: This antibody detects endogenous levels of total Histone H2A.X protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer.
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: H2A histone family member X
• Database Link:
  Entrez Gene 3014 Human
  UniProt ID P16104
• Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
  H2AX is an early responder to DNA damage, and its change to what is known as gamma-H2AX is important for the coordination of signaling and repair activities.
• Synonyms: H2a/x, H2AFX, H2AX