Myosin Light Chain 2 (MYL2) Rabbit Polyclonal Antibody

CAT#: AP02680PU-N

Myosin Light Chain 2 (MYL2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from293 and 3T3 cells using Myosin Light Chain 2 antibody

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  Immunofluorescence staining of methanol-fixed HeLa cells using Myosin Light Chain 2 antibody (Red).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/100-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around amino acids 17~21 (A-T-S-N-V) derived from Human Myosin Light Chain 2 Protein.
• Specificity: This antibody AP02680PU detects endogenous levels of total Myosin Light Chain 2 protein.
• Formulation: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 18 kDa (SDS-PAGE)
• Gene Name: myosin light chain 2
• Database Link:
  Entrez Gene 17906 MouseEntrez Gene 363925 RatEntrez Gene 4633 Human
  UniProt ID P10916
• Background: Myosin light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
• Synonyms: MLC-2v, MLC-2

Reference Data

• Protein Pathways: Cardiac muscle contraction, Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction