VEGF Receptor 2 (KDR) Rabbit Polyclonal Antibody

CAT#: AP02618PU-N

VEGF Receptor 2 (KDR) rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2 antibody (left) or the same antibody preincubated with Blocking Peptide (Right).

• 

  Figure 2. Immunofluorescence staining of Methanol-fixed MCF cells using this VEGFR2 antibody.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunofluorescence: 1/100 - 1/200.
  Immunohistochemistry on Paraffin Sections: 1/50 - 1/100.
  Western Blot.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around amino acids 1212~1216 (F-H-YP-D-N) derived from Human VEGFR2.
• Specificity: VEGFR2 antibody detects endogenous levels of total VEGFR2 protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: kinase insert domain receptor
• Database Link:
  Entrez Gene 3791 Human
  UniProt ID P35968
• Background: VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
• Synonyms: VEGFR2, FLK1, KDR, VEGF Receptor 2
• Note: Molecular Weight: 230 kDa