NFkB p100 / p52 (NFKB2) Rabbit Polyclonal Antibody

CAT#: AP02558PU-N

NFkB p100 / p52 (NFKB2) rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 3 Western Blot analyis of extracts from HeLa and HT29 cells using NFkappaB-p100/p52 antibody

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  Figure 1. Immunohistochemical analysis of Paraffin-Embedded Human breast carcinoma tissue using NF?B-p100/p52 antibody (Left) or the same antibody preincubated with Blocking peptide (Right)

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  Figure 2. Immunofluorescence NFkB-p100/p52 antibody staining of Methanol-fixed Hela cells.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunofluorescence: 1/100 - 1/200.
  Immunohistochemistry on Paraffin Sections: 1/50 - 1/100.
  Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide KLH conjugated around amino acids 864~868 (E-D-SP-A-Y) derived from Human NFkB-p100/p52
• Specificity: This antibody detects endogenous levels of NF-κB p100/p52 protein around serine 865.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: nuclear factor kappa B subunit 2
• Database Link:
  Entrez Gene 4791 Human
  UniProt ID Q00653
• Background: NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.
• Synonyms: LYT10, KBF2, H2TF1, NF-kB p100, NF-kB p52, NF kappa B