PDPK1 Rabbit Polyclonal Antibody
Other products for "PDPK1"
Specifications
Product Data | |
Applications | IF, IHC, WB |
Recommended Dilution | Western Blot: 1/500 - 1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry on paraffin sections: 1/50 - 1/100. Immunofluorescence: 1/100 - 1/200. |
Reactivities | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Synthetic non-phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241(A-N-SP-F-V). |
Specificity | PDK1 antibody detects endogenous levels of total PDK1 protein. |
Formulation | PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer. State: Aff - Purified State: Liquid purified IgG fraction |
Concentration | lot specific |
Purification | Affinity chromatography |
Conjugation | Unconjugated |
Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | 3-phosphoinositide dependent protein kinase 1 |
Database Link | |
Background | Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. |
Synonyms | PDK-1, PDK1, 3-phosphoinositide-dependent proteinkinase 1, hPDK1, PDPK1 |
Reference Data |
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