PDPK1 Rabbit Polyclonal Antibody

CAT#: AP02549PU-N

PDPK1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from MCF, 293 and PC12 cells using PDK1 antibody

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  Figure 2. Western blot analysis of extracts from MDA-MB-435 cells using PDK1 antibody.

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  Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PDK1 antibody.

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  Immunofluorescence staining of methanol-fixed MCF7 cells using PDK1 antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/1000.
  Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic non-phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241(A-N-SP-F-V).
• Specificity: PDK1 antibody detects endogenous levels of total PDK1 protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer.
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: 3-phosphoinositide dependent protein kinase 1
• Database Link:
  Entrez Gene 5170 Human
  UniProt ID O15530
• Background: Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
• Synonyms: PDK-1, PDK1, 3-phosphoinositide-dependent proteinkinase 1, hPDK1, PDPK1