SHC (SHC1) pTyr349 Rabbit Polyclonal Antibody

CAT#: AP02541PU-N

SHC (SHC1) pTyr349 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of extracts from 293 cells, using Shc1 Antibody (Lane 1, 2 and 3) and Shc1 (PTyr349) Antibody (Lane 4, 5 and 6).

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  Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using Shc1 pTyr349 antibody (Red).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Werstern Blot: 1/500~1/1000.
  Immunofluorescence: 1/100-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Shc1 around the phosphorylation site of Tyrosine 349 (H-Q-Yp-Y-N).
• Specificity: This antibody detects endogenous levels of Shc1 only when phosphorylated at Tyrosine 349.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: SHC adaptor protein 1
• Database Link:
  Entrez Gene 6464 Human
  UniProt ID P29353
• Background: The SHC gene encodes for a signaling and transforming protein containing Src homology 2 and 3 (SH2 and SH3) domains. The SHC gene encodes 2 widely expressed overlapping proteins of 46 and 52 kD, both containing a C-terminal SH2 domain. Adjacent to the SH2 region is a glycine and proline rich region. These 2 proteins differ in their N terminals. SHC proteins are involved in mitogenic signal transduction and act by coupling growth factor receptors to the RAS signaling pathway.
  The protein encoded by the SHC1 gene is thought to act as an adaptor in many signal transduction pathways.
• Synonyms: SHC-transforming protein 1, SHC-transforming protein A, SHC-transforming protein 3, SHCA