GSK3 beta (GSK3B) pTyr216 Rabbit Polyclonal Antibody

CAT#: AP02535PU-N

GSK3 beta (GSK3B) pTyr216 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 3. Western blot analysis of extract from 293 cells untreated or treated with PMA using GSK3B pTyr216 antibody.

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  Figure 1. Immunohistochemical analysis of Paraffin-embedded human breast carcinoma tissue, using GSK3B pTyr216 antibody (left) or the same antibody preincubated with blocking peptide (right).

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  Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using GSK3B pTyr216 antibody (Red).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500~1/1000.
  Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of tyrosine 279/216 (V-S-Y(p)-I-C) derived from Human GSK3α/β.
• Specificity: The antibody detects endogenous level of GSK3α/β only when phosphorylated at tyrosine 279/216.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: glycogen synthase kinase 3 beta
• Database Link:
  Entrez Gene 2932 Human
  UniProt ID P49841
• Background: Glycogen Synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. GSK3 has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control and oncogenesis. Two isoforms, alpha (GSK3A; OMIM 606784) and beta (GSK3B) show a high degree of amino acid homology within their catalytic domains. GSK3B is involved in energy metabolism, neuronal cell development and body pattern formation.
• Synonyms: GSK-3 beta, Glycogen synthase kinase-3 beta, GSK3B
• Note: Molecular Weight: 46 kDa, 51 kDa