CDK1 pTyr15 Rabbit Polyclonal Antibody

CAT#: AP02497PU-N

CDK1 pTyr15 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from COLO (Lane 1, 5) and HepG2 (Lane 2, 6) and K562 (Lane 3, 4, 7, 8) cells, using CDC2 antibody (Lane 1, 2, 3 and 4) and CDC2 (pTyr15) antibody (Lane 5, 6, 7 and 8).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 1:500~1:1000
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of tyrosine 15 (G-T-YP-G-V).
• Specificity: CDC2 antibody detects endogenous levels of CDC2 only when phosphorylated at tyrosine 15.
• Formulation: BPS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cyclin-dependent kinase 1
• Database Link:
  Entrez Gene 983 Human
  UniProt ID P06493
• Background: The cell division control protein cdc2, also known as cyclin dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.
• Synonyms: CDK1, CDC2, CDC28A, CDKN1, P34CDC2, p34 protein kinase