AMPK alpha 1 (PRKAA1) pSer487 Rabbit Polyclonal Antibody

CAT#: AP02451PU-N

AMPK alpha 1 (PRKAA1) pSer487 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extract from HeLa cells untreated or treated with heat shock (30 min), using AMPK alpha-1 pSer487 Antibody.

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  Figure 4. Western blot analysis of extract from HUVEC cells untreated or treated with UV using AMPK alpha-1 pSer487 Antibody.

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  Figure 1. Immunohistochemical analysis of paraffin- embedded human colon carcinoma tissue, using AMPK1 pSer487 Antibody.

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  Figure 3. Immunofluorescence staining of methanol-fixed HeLa cells using AMPK alpha-1 pSer487 Antibody (Red).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin-Embedded Sections 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human AMPK alpha-1 around the phosphorylation site of serine 487 (S-G- SP-V-S).
• Specificity: This antibody detects endogenous levels of AMPK1 only when phosphorylated at Serine 487
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: protein kinase AMP-activated catalytic subunit alpha 1
• Database Link:
  Entrez Gene 65248 RatEntrez Gene 105787 MouseEntrez Gene 5562 Human
  UniProt ID Q13131
• Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
• Synonyms: AMPK1, AMPK alpha-1 chain
• Note: Molecular Weight: 63 kDa

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, mTOR signaling pathway, Regulation of autophagy