ZAP70 pTyr319 Rabbit Polyclonal Antibody

CAT#: AP02441PU-N

ZAP70 pTyr319 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of extract from Jurkat cells, using Zap-70 antibody (Line 1 and 2) and Zap-70 (phospho-Tyr319) antibody (Line 3 and 4).

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  Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using Zap-70 (phospho-Tyr319) antibody (Red).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western Blot: 1/500 - 1/1000.; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunofluorescence: 1/100 - 1/200.
  
  
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human Zap-70 around the phosphorylation site of Tyrosine 319 (S-P-Yp-S-D).
• Specificity: This antibody detects endogenous levels of VEGFR2 only when phosphorylated at Tyrosine 1214.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: 70kd
• Gene Name: zeta chain of T cell receptor associated protein kinase 70kDa
• Database Link:
  Entrez Gene 7535 Human
  UniProt ID P43403
• Background: Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
• Synonyms: ZAP-70, ZAP 70, SRK