FOXO4 pSer197 Rabbit Polyclonal Antibody

CAT#: AP02424PU-S

FOXO4 pSer197 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis of extracts from 293 cells using AFX antibody (Lane 1 and 2) and AFX (phospho-Ser197) antibody (#, Lane 3 and 4).

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  Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using AFX (phospho-Ser197) antibody.

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  Figure 3. Immunofluorescence staining of methanol-fixed MCF7 cells using AFX (phospho-Ser197) antibody (Red).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human AFX around the phosphorylation site of serine 197 (A-A-SP-M-D).
• Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  AFX (phospho-Ser197) antibody detects endogenous levels of AFX only when phosphorylated at serine 197.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: forkhead box O4
• Database Link:
  Entrez Gene 4303 Human
  UniProt ID P98177
• Background: FOXO4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.
• Synonyms: Forkhead box protein O4