FOXO1 pSer256 Rabbit Polyclonal Antibody

CAT#: AP02405PU-N

FOXO1 pSer256 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis using FKHR antibody (Lane 1, 2, 3 and 4) and FKHR (phospho-Ser256) antibody (Lane 5, 6, 7 and 8).

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  Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using FKHR (phospho-Ser256) antibody.

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  Figure 3. Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR pSer256 antibody (Red).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human FKHR around the phosphorylation site of serine 256 (A-A-Sp-M-D).
• Specificity: This antibody detects endogenous levels of FKHR only when phosphorylated at Serine 256.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 78-82 kDa (Predicted)
• Gene Name: forkhead box O1
• Database Link:
  Entrez Gene 2308 Human
  UniProt ID Q12778
• Background: FOXO1A (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein which is a member of a subfamily of the forkhead homeotic gene family of transcription factors. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Chromosomal aberrations involving FOXO1A are a cause of rhabdomyosarcoma 2 (RMS2); also known as alveolar rhabdomyosarcoma.
• Synonyms: Forkhead Box Protein O1, FOXO1, FKHR