Phospholipase C gamma 1 (PLCG1) pTyr783 Rabbit Polyclonal Antibody

CAT#: AP02395PU-N

Phospholipase C gamma 1 (PLCG1) pTyr783 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of extract from HL60 cells, untreated or treated with EGF using PLCG1 antibody (Phospho-Tyr783).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of Tyrosine 783 (G-F-YP-V-E) derived from Human PLC-gamma-1 .
• Specificity: This antibody detects endogenous levels of PLC-gamma 1 only when phosphorylated at Tyrosine 783.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography using using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: phospholipase C gamma 1
• Database Link:
  Entrez Gene 5335 Human
  UniProt ID P19174
• Background: Phospholipase C gamma (PLCG) binds to receptor tyrosine kinases, e.g. EGFR, PDGFR, leading to phosphorylation of PLC-gamma at Tyrosine 771, 783 and 1245. Phosphorylation of Tyrosine 783 leads to enzymatic activation of PLC-gamma. PLC-gamma hydrolyzes phosphatidylinostitol-4,5-bisphosphate (PIP2), thus generating the second messengers inostitol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
• Synonyms: PLC1, Phospholipase C-gamma-1, PLC-gamma-1, PLC gamma1 Phospholipase C-II, PLC-II, PLC-148
• Note: Molecular Weight: 155 kDa