Her2 (ERBB2) pTyr1248 Rabbit Polyclonal Antibody

CAT#: AP02377PU-N

Her2 (ERBB2) pTyr1248 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from HepG2 cells treated with PMA and MCF cells treated with EGF, using CD340 / ERBB2 / HER2 Antibody (pTyr1248).

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue usind CD340 / ERBB2 / HER2 Antibody (pTyr1248) (left) or the same antibody preincubated with blocking peptide (right)

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  Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using CD340 / ERBB2 / HER2 Antibody (pTyr1248)

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  Immunofluorescence staining of methanol-fixed MCF7 cells using CD340 / ERBB2 / HER2 Antibody (pTyr1248)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from Human HER2 around the phosphorylation site of Tyrosine 1248 (P-E-Yp-L-G).
• Specificity: This antibody detects endogenous levels of HER2 only when phosphorylated at Tyrosine1248.
• Formulation: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: no BSA/AMINES, and no glycerol.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 185 kDa (Predicted)
• Gene Name: erb-b2 receptor tyrosine kinase 2
• Database Link:
  Entrez Gene 2064 Human
  UniProt ID P04626
• Background: ErbB 2 is a receptor tyrosine kinase of the ErbB 2 family. It is closely related instructure to the epidermal growth factor receptor. ErbB 2 oncoprotein is detectable in a proportion of breast and other adenocarconomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis.
• Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19