Estrogen Receptor 1 (ESR1) pSer106 Rabbit Polyclonal Antibody

CAT#: AP02369PU-N

Estrogen Receptor 1 (ESR1) pSer106 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from MCF7 cells untreated or treated with EGF using Estrogen Receptor-alpha (pSer106) antibody

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  Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Estrogen Receptor-alpha (pSer106) antibody (left) or the same antibody preincubated with blocking peptide (right)

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  Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-alpha (pSer106) Antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human Estrogen Receptor-alpha around the phosphorylation site of serine106 (S-P- SP-P-L).
• Specificity: Estrogen Receptor-alpha (phospho-Ser106) antibody detects endogenous levels of Estrogen Receptor-α only when phosphorylated at serine 106.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: estrogen receptor 1
• Database Link:
  Entrez Gene 2099 Human
  UniProt ID P03372
• Background: Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription.
  Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.
• Synonyms: ER alpha, Estradiol receptor, ESR1, ESR, NR3A1