p95 NBS1 (NBN) pSer343 Rabbit Polyclonal Antibody

CAT#: AP02356PU-N

p95 NBS1 (NBN) pSer343 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from Hela cells untreated or treated with Hydroxyurea using p95/NBS1 (phospho-Ser343) antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500-1/1000.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human p95/NBS1 around the phosphorylation site of Serine 343 (S-L-Sp-Q-G).
• Specificity: The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  This antibody detects endogenous levels of p95/NBS1 only when phosphorylated at Serine 343.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: nibrin
• Database Link:
  Entrez Gene 4683 Human
  UniProt ID O60934
• Background: The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex (MRN complex). This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions. In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells. NBS1 is expressed ubiquitously and presents high levels in testis.
• Synonyms: NBN, NBS, NBS1, P95
• Note: Molecular Weight: 95 kDa