c-Myc (MYC) pThr358 Rabbit Polyclonal Antibody

CAT#: AP02336PU-S

c-Myc (MYC) pThr358 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from HT29 cells untreated (Lane 1) or treated with UV (Lane 2) using Myc (pThr358) Antibody

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  Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using Myc (pThr358) Antibody (left) or the same Antibody preincubated with blocking peptide (right)

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  Immunofluorescence staining of methanol-fixed HeLa cells using Myc (pThr358) Antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human Myc around the phosphorylation site of threonine 358 (R-R-TP-H-N).
• Specificity: Myc (phospho-Thr358) antibody detects endogenous levels of Myc only when phosphorylated at threonine 358.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02% Sodium Azide as preservative and 50% glycerol as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: v-myc avian myelocytomatosis viral oncogene homolog
• Database Link:
  Entrez Gene 4609 Human
  UniProt ID P01106
• Synonyms: Transcription factor p64, BHLHE39