c-Myc (MYC) pThr58 Rabbit Polyclonal Antibody

CAT#: AP02335PU-S

c-Myc (MYC) pThr58 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (phospho-Thr58) antibody.

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  Figure 2. Western blot analysis from HeLa cells using Myc (phospho-Thr58) antibody and the same antibody with blocking peptide

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Suitable for use in Western blot (1:500~1:1000) and Immunohistochemistry (1:50~1:100).
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Myc around the phosphorylation site of threonine 58 (L-P-TP-P-P).
• Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  Myc (phospho-Thr58) antibody detects endogenous levels of Myc only when phosphorylated at threonine 58.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: v-myc avian myelocytomatosis viral oncogene homolog
• Database Link:
  Entrez Gene 4609 Human
  UniProt ID P01106
• Synonyms: Transcription factor p64, BHLHE39