c-Jun (JUN) pThr239 Rabbit Polyclonal Antibody

CAT#: AP02325PU-S

c-Jun (JUN) pThr239 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from HeLa cells untreated or treated with UV using c-Jun (pThr239) Antibody

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  Immunohistochemical analysis of paraffin-embedded human breast cacinoma tissue using c-Jun (pThr239) Antibody (left) or the same antibody preincubated with blocking peptide (right)

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  Immunofluorescence staining of methanol-fexed HeLa cells using c-Jun (pThr239) Antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of threonine 239 (G-E-TP-P-P).
• Specificity: c-Jun (phospho-Thr239) antibody detects endogenous levels of c-Jun only when phosphorylated at threonine 239.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02% Sodium Azide as preservative and 50% glycerol as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Jun proto-oncogene, AP-1 transcription factor subunit
• Database Link:
  Entrez Gene 3725 Human
  UniProt ID P05412
• Background: The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun (previously known as the Fos binding protein p39) and c Fos form a complex in the nucleus. AP 1 (activating protein 1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies.
• Synonyms: Transcription factor AP1