MEK2 (MAP2K2) pThr394 Rabbit Polyclonal Antibody

CAT#: AP02310PU-N

MEK2 (MAP2K2) pThr394 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracted from HepG2 and HeLa untreated or treated with UV using MEK-2 (pThr394) antibody

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  Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using MEK-2 (pThr394) antibody (left) or the same antibody preincubated with blocking peptide (right)

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  Immunofluorescence staining of mehtanol fixed HeLa cells using MEK-2 (pThr394) Antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human MEK2 around the phosphorylation site of threonine 394 (P-G-TP-P-T).
• Specificity: MEK2 (phospho-Thr394) antibody detects endogenous levels of MEK2 only when phosphorylated at threonine 394.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02% Sodium Azide as preservative and 50% glycerol as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: 44 kDa
• Gene Name: mitogen-activated protein kinase kinase 2
• Database Link:
  Entrez Gene 5605 Human
  UniProt ID P36507
• Background: Microtubule associated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK 1 & 2/MAPK enzymes by phosphorylating both residues within the threonine/glutamic acid/tyrosine (TEY) motif in the activation loop. MEK 1 & 2 are also activated by dual phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs. Threonine 394 of MEK2 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK2.
• Synonyms: MAP kinase kinase 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK2, MKK2, MAP kinase kinase 2