RAF1 pSer259 Rabbit Polyclonal Antibody

CAT#: AP02308PU-S

RAF1 pSer259 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 2. Western blot analysis using Raf-1 antibody (Line 1, 2, and 3) and Raf-1 (phospho-Ser259) antibody (Line 4, 5, and 6).

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  Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf-1 (phospho-Ser259) antibody

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot (1:500-1:1000).
  Immunohistochemistry (1:50-1:100).
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Raf-1 around the phosphorylation site of serine 259 (S-T-Sp-T-P).
• Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  Raf-1 (phospho-Ser259) antibody detects endogenous levels of Raf-1 only when phosphorylated at serine 259.
• Formulation: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% glycerol.
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Raf-1 proto-oncogene, serine/threonine kinase
• Database Link:
  Entrez Gene 5894 Human
  UniProt ID P04049
• Synonyms: C-RAF, Raf-1, cRaf