VEGFA Rabbit Polyclonal Antibody

CAT#: AP02022SU-N

VEGFA rabbit polyclonal antibody, Serum

Product Images

Western Blot analysis AP02022SU VEGF antibody specificity. Differenet recombinant Human (rh) and Recombinant Bovine (rb) VEGF proteins were separated by SDS-PAGE and immunoblotted with AP02022SU (1/10,000). Lane 1: rhVEGF165 (200ng). Lane 2: rhVEGF189 (cell lysate). Lane 3: rbVEGF164 (50ng). Lane 4: rbVEGF164 (100ng). Lane 5: rbVEGF164 (100ng) with AP02022SU preadsorbed with 15 mg/ml of rhVEGF165. Lane M: Rainbow Marker. Berisha B et al. (2000) Biol. Reprod 63 (4): 1106-14.

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  Immunohistochemistry of VEGF staining of Paraffin Sections of Bovine follicules using AP02022SU VEGF antibody. Antigen retrieval was performed by heating the dewaxed sections 4x to 95°C for 5min. The section was then treated with Hydrogen Peroxide (1%) in methanol for 30 min to block endogenous peroxidase. The section was incubated with AP02022SU (1/300) overnight, followed by staining with ABC method. DAB in 0.0006% Hydogen Peroxide/0.05M Tris buffer (pH7.6) was used as the chromogen. This antibody stains the cytoplasm of granulosa and theca cells of mature follicules. Scale bar: 100 µm. Berisha B et al. (2002) J. Endocrinol. 167 (3): 371-382.

Specifications

Product Data

• Applications: IHC, R, WB
• Recommended Dilution: Radioimmunoassay (1/400,000).
  Western blot (1/10,000).
  Immunohistochemistry on CryoSections (1/2,000).
  Immunohistochemistry on Paraffin Sections (1/300).
  See also References 1-5 for more details.
• Reactivities: Bovine, Human, Porcine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant Bovine Vascular Endothelial Growth Factor-164.
• Specificity: This antibody recognizes Bovine, Porcine domestic ruminants VEGF-164 and detects all Human VEGF isoforms (121, 165, 189, and 206).
  No cross reaction was obtained with PDGF-AA, PDGF-BB, PDGF-AB, FGF-1, FGF-2, and TGF-alpha.
• Formulation: State: Serum
  State: Lyophilized Serum
• Reconstitution Method: Restore in aqua bidest to initial volume.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 281572 Bovine
  UniProt ID P15691
• Background: Vascular endothelial growth factor (VEGF) is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels.
• Synonyms: VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor
• Note: LocusID 7124