NF-kB p65 (RELA) pThr254 Rabbit Polyclonal Antibody

CAT#: AP01651PU-N

NF-kB p65 (RELA) pThr254 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of NF?B-p65 pThr254 antibody in extracts from 293 cells treated with TNF-alpha.

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  Immunohistochemistry (IHC) analysis of NF?B-p65 pThr254 antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA: 1/20000-1/40000.
  Western Blot: 1/500-1/1000.
  Immunohistochemistry: 1/50-1/200.
  Immunoprecipitation: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of NFκB-p65 pThr254 protein.
• Formulation: Phosphate buffered saline (PBS), pH~7.2 containing 15 mM Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: RELA proto-oncogene, NF-kB subunit
• Database Link:
  Entrez Gene 5970 Human
  UniProt ID Q04206
• Background: The NFκB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The DNA binding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.
• Synonyms: NF kappa B p65, NFkB p65, Transcription factor p65, Rel A, NFKB3