NF-kB p65 (RELA) pSer536 Rabbit Polyclonal Antibody

CAT#: AP01650PU-N

NF-kB p65 (RELA) pSer536 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p-NF?B-p65 antibody (Cat.-No.: AP01650PU-N) at 1/500 dilution. Lane 1: Hela cell lysate treated with UV, Lane 2: Mouse kidney tissue lysate

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  Immunohistochemistry (IHC) analysis of NF?B-p65 pSer536 Antibody (Cat.-No.: AP01650PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunoprecipitation: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of NFκB-p65 pSer536 protein.
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~ 60,75 kDa
• Gene Name: RELA proto-oncogene, NF-kB subunit
• Database Link:
  Entrez Gene 5970 Human
  UniProt ID Q04206
• Background: p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory prd in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibittransforming activity.
• Synonyms: NF kappa B p65, NFkB p65, Transcription factor p65, Rel A, NFKB3