IKK alpha (CHUK) pThr23 Rabbit Polyclonal Antibody

CAT#: AP01612PU-N

IKK alpha (CHUK) pThr23 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of IKK-alpha pThr23 antibody in extracts from MDA-MB-435 cells treated with EGF.

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  Immunohistochemistry (IHC) analysis of IKK-alpha pThr23 antibody in paraffin-embedded human colon carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/5000-1/10000.
  Western Blot: 1/500-1/1000.
  Immunohistochemistry: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of IKK-alpha pThr23 protein.
• Formulation: Phosphate buffered saline (PBS), pH~7.2 containing 15 mM Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: conserved helix-loop-helix ubiquitous kinase
• Database Link:
  Entrez Gene 1147 Human
  UniProt ID O15111
• Background: Nuclear factor kappa B (NFkB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NFkB mediates the expression of a great variety of genes in response to extracellular stimuli including IL1, TNF alpha, and bacterial product LPS. NFkB is associated with IkB proteins in the cell cytoplasm, which inhibit NFkB activity. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKK alpha and IKK beta). IKK alpha and IKK beta interact with each other and both are essential for NFkB activation. IKK alpha specifically phosphorylates IkBa. IKKa is expressed in variety of human tissues.
• Synonyms: CHUK, TCF16, I kappa-B kinase alpha, IkBKA, IKK-alpha, IKK-A, IkappaB kinase, I-kappa-B kinase 1, NFKBIKA, IKK1