IGF1 Receptor (IGF1R) pTyr1161 Rabbit Polyclonal Antibody

CAT#: AP01610PU-N

IGF1 Receptor (IGF1R) pTyr1161 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of IGF-1R pTyr1161 antibody in extracts from 293 cells treated with Insulin.

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  Immunohistochemistry (IHC) analysis of IGF-1R pTyr1161 antibody in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: Peptide ELISA: 1/20000-1/40000.
  Western Blot: 1/500-1/1000.
  Immunohistochemistry: 1/50-1/200.
  Immunoprecipitation: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human IGF-1R around the phosphorylation site of Tyrosine 1161.
• Specificity: This antibody detects endogenous levels of IGF-1R pTyr1161 protein.
• Formulation: Phosphate buffered saline (PBS), pH~7.2 containing 15 mM Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~ 95, 155 kDa
• Gene Name: insulin like growth factor 1 receptor
• Database Link:
  Entrez Gene 3480 Human
  UniProt ID P08069
• Background: There are two kinds of IGF receptors based on their relative affinities for IGF1 versus IGF2. The IGF1 receptor prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kD) and two beta (95 kD) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell surviva.
• Synonyms: IGF-I receptor, IGF1 Receptor