MARCKS Rabbit Polyclonal Antibody

CAT#: AP01344PU-N

MARCKS rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of MARCKS antibody (Cat.-No.: AP01344PU-N) in extracts from mouse brain cells.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: MARCKS antibody detects endogenous levels of MARCKS protein. (region surrounding Lys152)
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 45 kDa
• Gene Name: myristoylated alanine rich protein kinase C substrate
• Database Link:
  Entrez Gene 4082 Human
  UniProt ID P29966
• Background: Myristoylated alanine-rich protein kinase C substrate (MARCKS), also designated 80K or 80K-L, has been identified as a major cellular substrate for protein kinase C. Human MARCKS is a 332 amino acid protein with a calculated molecular weight of 31.534 kDa; however, it has been shown to run at 80-87 kDa on Western blot. The plasma membrane bound protein dissociates from the membrane upon phosphorylation by various PKC isoforms. In NIH/3T3 fibroblasts, PKC α and PKC ε, but not PKC δ, are responsible for MARCKS phosphorylation. MARCKS has been found to bind calmodulin, Actin and Synapsin and is a filamentous (F) Actin crosslinking protein.
• Synonyms: Myristoylated alanine-rich C-kinase substrate, MACS, PRKCSL