EMR2 (ADGRE2) Rabbit Polyclonal Antibody

CAT#: AP01214PU-N

EMR2 (ADGRE2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of EMR2 antibody in extracts from HeLa cells.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immnofluorescence: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 130-178 of Human EMR2.
• Specificity: This antibody detects endogenous levels of EMR2 protein. (region surrounding Leu160)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~ 90 kDa
• Gene Name: adhesion G protein-coupled receptor E2
• Database Link:
  Entrez Gene 30817 Human
  UniProt ID Q9UHX3
• Background: EMR2 is a member of the class B seven-span transmembrane (TM7) receptor family expressed predominantly by cells of the immune system, where it has been shown to be restricted specifically to monocytes and granulocytes. Family members are characterized by an extended extracellular region with a variable number of N terminal epidermal growth factor (EGF)-like domains coupled to a TM7 domain via a mucin-like spacer domain. This gene is closely linked to the gene encoding egf-like molecule containing mucin-like hormone receptor 3 on chromosome 19. This protein does not interact with the ligand decay accelerating factor for complement, unlike the related CD97 antigen, and indicates that these very closely related proteins likely have nonredundant functions. This gene results in multiple transcripts encoding distinct isoforms. ESTs have been isolated from blood, colon, lymph node, placenta, spleen, testis, and uterus libraries.