IL17B Rabbit Polyclonal Antibody

CAT#: AP01148BT-N

IL17B rabbit polyclonal antibody, Biotin

Product Images

Western Blot (Unreduced) using Biotin conjugated anti-Interleukin-17B / IL17B Antibody Cat.-No AP01148BT

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  Western Blot (Reduced) using Biotin conjugated anti-Interleukin-17B / IL17B Antibody Cat.-No AP01148BT

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  Direct ELISA using Biotin conjugated anti-Interleukin-17B / IL17B Antibody Cat.-No AP01148BT

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  Sandwich ELISA using Biotin conjugated anti-Interleukin-17B / IL17B Antibody Cat.-No AP01148BT

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect hIL-17B (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17B.
  Sandwich ELISA: To detect hIL-17B (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human IL-17B (Cat.-No AP01148PU) as a capture antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17B.
  Western blot: To detect hIL-17B this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17B is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98%) E.coli derived recombinant Human IL-17 beta (Cat.-No PA283)
• Specificity: This antibody detects Human Interleukin-17 beta. Other species not tested.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Lyophilized purified (sterile filtered) Ig fraction
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Antigen Affinity Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: interleukin 17B
• Database Link:
  Entrez Gene 27190 Human
  UniProt ID Q9UHF5
• Background: IL17B is a non disulfide-linked homodimer of two 161 amino acid polypeptide chains. It belongs to the IL17 family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL17A through IL17F, are secreted as homodimers. IL17B is expressed by T-cells and has been shown to stimulate release of TNF-alpha and IL-1beta from cells of the monocyte lineage.
• Synonyms: IL-17B, IL20, NIRF, ZCYTO7, Neuronal interleukin-17-related factor, Interleukin-20
• Note: Centrifuge vial prior to opening.

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction