IL28A (IFNL2) Rabbit Polyclonal Antibody

CAT#: AP01147PU-N

IL28A (IFNL2) rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Indirect ELISA: To detect hIFN-λ2 (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIFN-λ2.
  Sandwich ELISA: To detect hIFN-λ2 (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IFN-λ2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIFN-λ2.
  Western blot: To detect hIFN-λ2 this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-λ2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  Immunostaining.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98%)E.coli derived, 19.6 kDa recombinant human IFN-λ2
• Specificity: This antibody det cts Interferon-lambda 2.
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Sterile filtered lyophilized Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/ml.
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: interferon, lambda 2
• Database Link:
  Entrez Gene 282616 Human
  UniProt ID Q8IZJ0
• Background: IL28 is a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA).
• Synonyms: IL-28A, Interferon lambda-2, IFNL2, ZCYTO20

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway