CXCL14 Rabbit Polyclonal Antibody

CAT#: AP01141BT-S

CXCL14 rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: Direct: To detect hBRAK (using 100 μl/well antibody solution) a concentration of
  0.25 - 1.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBRAK.
  Sandwich: To detect hBRAK (using 100 μl/well antibody solution) a concentration of
  0.25 - 1.0 μg/ml of this antibody is required. This In conjunction with Polyclonal Anti-Human BRAK as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBRAK.
  Western blot: To detect hBRAK this antibody can be used at a concentration of
  0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBRAK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98 %) recombinant human BRAK
• Specificity: This antibody detects BRAK.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Sterile filtered lyophilized Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restoree in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/ml.
• Purification: Affinity chromatography
• Conjugation: Biotin
• Storage: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at
  2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: C-X-C motif chemokine ligand 14
• Database Link:
  Entrez Gene 9547 Human
  UniProt ID O95715
• Background: BRAK/CXCL14 is a CXC chemokine constitutively expressed at the mRNA level in certain normal tissues but absent from many established tumor cell lines and human cancers. Although multiple investigators have cloned BRAK, little is known regarding the physiologic function of BRAK or the reason for decreased expression in cancer.
• Synonyms: NJAC, SCYB14