NNT1 (CLCF1) Rabbit Polyclonal Antibody

CAT#: AP01128PU-S

NNT1 (CLCF1) rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Indirect ELISA: To detect hNNT-1/BCSF-3 by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1/BCSF-3.
  Sandwich ELISA: To detect hNNT-1/BCSF-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human NNT-1/BCSF-3 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1/BCSF-3.
  Western Blot: To detect hNNT-1/BCSF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNNT-1/BCSF-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98%) E.coli derived 22.4 kDa recombinant human NNT-1/BCSF-3
• Specificity: This antibody detects NNT-1/BCSF-3.
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Sterile filtered lyophilized Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1 - 1.0 mg/ml.
• Purification: Immunoaffinity chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cardiotrophin-like cytokine factor 1
• Database Link:
  Entrez Gene 23529 Human
  UniProt ID Q9UBD9
• Background: NNT1 belongs to the interleukin 6 family of cytokines, which are involved in cell signaling through phosphorylation of gp130. IL6 family members share similarity in gene structure and have a 4 helix bundle in their protein structure.
  NNT1 has a sequence of 225-amino acids with a 27-aa signal peptide, a molecular mass of 22 kDa in mature form, and the highest homology to cardiotrophin-1 and ciliary neurotrophic factor. NNT1 may act as a neuroimmunoendocrine modulator of hypothalamus-pituitary-adrenal axis stress response.
• Synonyms: BSF3, CLC, NNT1, Novel neurotrophin-1
• Note: Centrifuge vial prior to opening.

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway